ITGB4/Integrin Beta 4 Antibody

Product Specifications

Product Name Alternative

ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp150, CD104 antigen, Integrin beta-4 subunit, Integrin, beta 4

Reactivity

Human

Immunogen

Human ITGB4

Target

ITGB4 / Integrin Beta 4

Clonality

Monoclonal

Clone

439-9B

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Affinity purified

Concentration

0.5 mg/ml

Dilution

Flo, IHC, IHC-Fr, IHC-P (20 μg/ml), WB

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: This antibody is for use in Western Blot only under non-reducing conditions. Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20 ug/ml. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.