ITGAM/CD11b Antibody

Product Specifications

Product Name Alternative

ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

Reactivity

Human, Mouse, Rabbit

Immunogen

T cell enriched splenocytes from B10 mice

Target

ITGAM / CD11b

Clonality

Monoclonal

Clone

M1/70.15

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Ion exchange chromatography

Concentration

1 mg/ml

Dilution

Flo (1:50), IF, IHC, IHC-Fr, IHC-P (10 μg/ml), IP

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.