DAP12 Antibody
DAP12 Antibody
Product Specifications
Product Name Alternative
TYROBP, DNAX-activation protein 12, DAP12, PLOSL, KARAP, KAR-associated protein, PLO-SL
Reactivity
Canine, Equine, Human, Mammal, Monkey
Immunogen
Synthetic peptide C-KQRITETESPYQE from an internal region of human TYROBP / DAP12 (NP_003323.1; NP_937758.1) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%) ; Sheep, Panda, Rabbit (92%) ; Mouse, Elephant, Zebu, Bovine (85%) .
Target
DAP12
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Immunoaffinity purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (3 - 6 μg/ml), Peptide-ELISA
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 3-6 ug/ml.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
50207121 3x 1.0µg DNA
VSIG4 CRISPRa sgRNA lentivector (set of three targets)(Human)
Sign In for Pricing
View Details 