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PDK1 Antibody

PDK1 Antibody

Product Specifications

Product Name Alternative

PDK1, PDHK, PDH kinase 1, PDHK1

Reactivity

Canine, Human, Monkey, Mouse, Porcine, Rabbit, Rat

Immunogen

Synthetic peptide derived from the sequence of human PDK1, conjugated to KLH.

Target

PDK1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein A purified

Concentration

1 mg/ml

Dilution

IHC, IHC-P (10 μg/ml), WB (1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml

Tested Applications

IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS, 0.09% Sodium Azide, 50% Glycerol

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