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UBC/Ubiquitin C Antibody

UBC/Ubiquitin C Antibody

Product Specifications

Product Name Alternative

UBC, HUBCEP52, L40, HMG20, RPL40, Ubiquitin, Polyubiquitin-C, CEP52, Ubiquitin C

Reactivity

Human

Target

UBC / Ubiquitin C

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Ammonium sulfate precipitation

Concentration

2 mg/ml

Dilution

IF (1:10 - 1:50), IHC, IHC-P (10 μg/ml), WB (1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.

Tested Applications

IF, IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS, 0.09% Sodium Azide

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