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RETNLB/RELM-Beta Antibody

RETNLB/RELM-Beta Antibody

Product Specifications

Product Name Alternative

RETNLB, CCRG, Fizz1, HXCP2, Found in inflammatory zone 1, Resistin like beta, Resistin-like beta, RELM-beta, RELMb, RELMbeta, RETNL2, FIZZ2, Xcp2

Reactivity

Human

Immunogen

Peptide with sequence C-DSVMDKKIKDVLNS, from the internal region of the protein sequence according to NP_115968.1.

Target

RETNLB / RELM-Beta

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (2.5 μg/ml), Peptide-ELISA (1:128000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave an approx 35kD band in Human Ileum and Duodenum lysates after 0.1 ug/ml antibody staining.

Tested Applications

ELISA, IHC, IHC-P

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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