KRT13/CK13/Cytokeratin 13 Antibody

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 55kD band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kD according to NP_705694.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149 (Pt 4) :961-71.; PMID: 12686638) . Recommended concentration: 0.1-0.3 ug/ml.