CUL3/Cullin 3 Antibody
CUL3/Cullin 3 Antibody
Product Specifications
Product Name Alternative
CUL3, Cullin 3, Cullin homolog 3, Cullin-3, KIAA0617, PHA2E, CUL-3
Reactivity
Human, Mouse
Immunogen
Cul3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal near amino acids 1-25 of Human Cul3 coupled to KLH.
Target
CUL3 / Cullin 3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Delipidated and defibrinated followed by sterile filtration
Concentration
85 mg/ml
Dilution
ELISA (1:2000 - 1:10000), IHC, IHC-P (1:500), IP (1:100), WB (1:500 - 1:1000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:500.
Tested Applications
ELISA, IHC, IHC-P, IP, WB
Host or Source
Rabbit
Preservative
Antiserum, 0.01% Sodium Azide
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