RNH1 Antibody
RNH1 Antibody
Product Specifications
Product Name Alternative
RNH1, Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH
Reactivity
Human, Mouse
Immunogen
Recombinant Ribonuclease inhibitor 1 (RNH1) (7-461aa) purified from E. coli
Target
RNH1
Clonality
Monoclonal
Clone
AT1H23
Conjugation
Unconjugated
Purification
Protein G purified
Concentration
1 mg/ml
Dilution
ELISA, IHC, IHC-P (5 μg/ml), WB (1:1000 - 1:2000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Extracts of mouse liver (20ug) were resolved by This, transferred to NC membrane and probed with anti-human RNH1 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Mouse
Preservative
PBS, pH 7.4, 0.1% Sodium Azide
Isotype
IgG2a, k
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