NR1I3/CAR Antibody
NR1I3/CAR Antibody
Product Specifications
Product Name Alternative
NR1I3, Car-beta, CAR1, Constitutive active receptor, Constitutive active response, Orphan nuclear receptor MB67, MB67, CAR
Reactivity
Human
Immunogen
Synthetic peptide located between aa180-229 of human NR1I3 (Q6GZ85, NP_001070939) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Medaka (100%) ; Zebrafish (85%) ; Hamster, Turkey, Zebra finch, Chicken, Platypus, Lizard, Stickleback (84%) .
Target
NR1I3 / CAR
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Immunoaffinity purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (5 μg/ml), WB (0.2 - 1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
PBS, 0.09% sodium azide, 2% sucrose
Isotype
IgG
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