LDOC1 Antibody

Product Specifications

Product Name Alternative

LDOC1, Breast cancer, up-regulated 1, Mart7, BCUR1, Mar7, Protein LDOC1

Reactivity

Human

Immunogen

Synthetic peptide from N-Terminus of human LDOC1 (O95751, NP_036449) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Bovine, Rabbit, Pig, Guinea pig (100%) ; Monkey, Bat, Horse (92%) ; Dog (85%) .

Target

LDOC1

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Immunoaffinity purified

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), WB (0.2 - 1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.