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NR1H3/LXR Alpha Antibody

NR1H3/LXR Alpha Antibody

Product Specifications

Product Name Alternative

NR1H3, Liver X receptor alpha, LXRA, LXR-a, Oxysterols receptor LXR-alpha, LXR alpha, Lxralpha, RLD-1

Reactivity

Human, Mouse

Immunogen

Synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%) .

Target

NR1H3 / LXR Alpha

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Immunoaffinity purified

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), WB

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Tested Applications

IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS, 0.09% sodium azide, 2% sucrose

Isotype

IgG

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