PAX4 Antibody

Product Specifications

Product Name Alternative

PAX4, KPD, Paired box protein Pax-4, Paired domain gene 4, Paired box 4, Paired box gene 4, MODY9

Reactivity

Human, Rat

Immunogen

Synthetic peptide located between aa166-215 of human PAX4 (A4D0Z1, NP_006184) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey (100%) ; Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (92%) .

Target

PAX4

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Immunoaffinity purified

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), WB (0.2 - 1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.