NR0B1/DAX1 Antibody

NR0B1, AHX, AHC, AHCH, DAX1, DSS, DAX 1, DAX-1, Adrenal hypoplasia congenita, Dosage-sensitive sex reversal, GTD, HHG, Nuclear hormone receptor, NROB1, Nuclear receptor DAX-1, SRXY2, Nuclear receptor DAX1, Dax-1 receptor

Synthetic peptide located between aa359-408 of human NR0B1 (Q9BG96, NP_000466) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%) ; Galago, Hamster, Xenopus, Seabass, Salmon, Stickleback, Medaka (92%) ; Bat (91%) ; Zebrafish (85%) ; Catfish (81%) .

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.