PECAM-1/CD31 Antibody
PECAM-1/CD31 Antibody
Product Specifications
Product Name Alternative
PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA
Reactivity
Human
Immunogen
Purified recombinant fragment of human CD31 expressed in E. Coli.
Target
PECAM-1 / CD31
Clonality
Monoclonal
Clone
2F7B2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Ascites
Concentration
1 mg/ml
Dilution
ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200), WB (1:100 - 1:500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:200.
Tested Applications
ELISA, IF, IHC, IHC-P, WB
Host or Source
Mouse
Preservative
Ascitic fluid, 0.03% sodium azide
Isotype
IgG1
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