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S100A10 Antibody

S100A10 Antibody

Product Specifications

Product Name Alternative

S100A10, 42C, ANX2LG, Calpactin-1 light chain, Calpactin I light chain, CLP11, p11, p10, p10 protein, Protein S100-A10, ANX2L, Ca[1], CAL1L, Cellular ligand of annexin II, gp11

Reactivity

Human

Immunogen

Purified recombinant fragment of human P11 expressed in E. Coli.

Target

S100A10

Clonality

Monoclonal

Clone

4E7E10

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein A purified

Concentration

1 mg/ml

Dilution

ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200), WB (1:100 - 1:500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:200.

Tested Applications

ELISA, IF, IHC, IHC-P, WB

Host or Source

Mouse

Preservative

Ascitic fluid, 0.03% sodium azide

Isotype

IgG1

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