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ICAM-1/CD54 Antibody

ICAM-1/CD54 Antibody

Product Specifications

Product Name Alternative

ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

Reactivity

Human

Immunogen

Human monocytes

Target

ICAM-1 / CD54

Clonality

Monoclonal

Clone

15.2

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G purified

Concentration

1 mg/ml

Dilution

Flo (1:50 - 1:100), IHC, IHC-Fr (1:50 - 1:100), IHC-P (10 μg/ml), IP

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.

Tested Applications

FC, IHC, IHC-Fr, IHC-P, IP

Host or Source

Mouse

Preservative

PBS, 0.09% Sodium Azide

Isotype

IgG1

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