ICAM-1/CD54 Antibody
ICAM-1/CD54 Antibody
Product Specifications
Product Name Alternative
ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58
Reactivity
Human
Immunogen
Human monocytes
Target
ICAM-1 / CD54
Clonality
Monoclonal
Clone
15.2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G purified
Concentration
1 mg/ml
Dilution
Flo (1:50 - 1:100), IHC, IHC-Fr (1:50 - 1:100), IHC-P (10 μg/ml), IP
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.
Tested Applications
FC, IHC, IHC-Fr, IHC-P, IP
Host or Source
Mouse
Preservative
PBS, 0.09% Sodium Azide
Isotype
IgG1
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