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CD27 Antibody (FITC)

CD27 Antibody (FITC)

Product Specifications

Product Name Alternative

CD27, CD27 antigen, CD27 molecule, S152, T14, T-cell activation antigen CD27, CD27L receptor, T cell activation antigen S152, TNFRSF7, Tp55

Reactivity

Human, Mouse

Immunogen

MCD27 tag expressing clones AR0524 and AR0530

Target

CD27

Clonality

Monoclonal

Clone

LG.3A10

Conjugation

FITC

Field of Research

Immunology & Inflammation; Cell Biology

Purification

Protein G purified from ascites

Concentration

0.1 mg/ml

Dilution

Flo (0.5 μg/10E6 cells), IHC, IHC-Fr, IHC-P (10 μg/ml)

Storage Conditions

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Tested Applications

FC, IHC, IHC-Fr, IHC-P

Host or Source

Hamster

Preservative

PBS, 0.02% Sodium Azide, rAlbumin

Isotype

IgG1

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