NFATC1/NFAT2 Antibody
NFATC1/NFAT2 Antibody
Product Specifications
Product Name Alternative
NFATC1, NFATc, NFAT2, NF-ATC, NF-ATC transcription factor, NF-ATc1
Reactivity
Human
Immunogen
Recombinant human NFATc1 (428-716aa) purified from E. coli. NCBI Accession No.: NP_765978.
Target
NFATC1 / NFAT2
Clonality
Monoclonal
Clone
AT1C3
Conjugation
Unconjugated
Purification
Protein G purified
Concentration
1 mg/ml
Dilution
ELISA, IF, IHC, IHC-P (10 μg/ml), SDS-PAGE, WB (1:1000 - 1:2000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.
Tested Applications
ELISA, IF, IHC, IHC-P, SDS-PAGE, WB
Host or Source
Mouse
Preservative
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Isotype
IgG2a, k
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