PCK1 Antibody
PCK1 Antibody
Product Specifications
Product Name Alternative
PCK1, PEPCKC, Phosphopyruvate carboxylase, PEP carboxykinase, PEPCK-C, PEPCK1
Reactivity
Canine, Equine, Human, Mammal, Monkey, Rabbit
Immunogen
Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.
Target
PCK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (2 μg/ml), Peptide-ELISA (1:8000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2 ug/ml.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
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