FTH1/Ferritin Heavy Chain Antibody

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 22kD band observed in Human Brain (Cerebellum), Liver and Placenta lysates (calculated MW of 21.2kD according to NP_002023.2) . Recommended concentration: 0.03-0.1 ug/ml.