F8/FVIII/Factor VIII Antibody
F8/FVIII/Factor VIII Antibody. FVIII is a large molecule glycoprotein composed of 2,332 amino acids and is divided into multiple functional domains (arranged in the order of A1-A2-B-A3-C1-C2) .
Product Specifications
Product Name Alternative
F8, AHF, Antihemophilic factor, Coagulation factor VIIIc, Coagulation factor VIII, DXS1253E, Factor VIII F8B, FVIII, HEMA, F8B, Factor VIII, Procoagulant component, F8C
Reactivity
Human
Immunogen
Human Factor VIIIC (F. VIII) purified from F. VIII concentrate.
Target
F8 / FVIII / Factor VIII
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Caprylic acid and ammonium sulfate precipitations and solid-phase adsorption
Concentration
10 mg/ml
Dilution
ELISA, IHC, IHC-P (5 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Sheep
Preservative
10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol
Isotype
IgG
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