PDPN/Podoplanin Antibody
PDPN/Podoplanin Antibody
Product Specifications
Product Name Alternative
PDPN, AGGRUS, HT1alpha-1, Glycoprotein 36, Glycoprotein, 36-KD, gp36, gp38, OTS8, PA2.26, PA2.26 antigen, Podoplanin, T1A, gp40, HT1A-1, HT1alpha-2, p38, T1-alpha, T1A-2
Reactivity
Human, Mouse
Immunogen
Recombinant human PDPN (1-206aa) purified from E. coli.
Target
PDPN / Podoplanin
Clonality
Monoclonal
Clone
5E2
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Protein G purified
Concentration
1 mg/ml
Dilution
ELISA, IHC, IHC-P (5 μg/ml), WB (1:500 - 1:1000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Mouse
Preservative
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Isotype
IgG2b, k
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