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IL1R1 Antibody

IL1R1 Antibody

Product Specifications

Product Name Alternative

IL1R1, Antigen CD121a, D2S1473, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1R, IL1RA, IL1RT1, Interleukin receptor 1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, CD121A, CD121a antigen, Interleukin 1 receptor, type I, Interleukin-1 receptor type I, Interleukin-1 type i receptor, p80

Reactivity

Human

Immunogen

The extracellular domain of the type I human IL-1 receptor.

Target

IL1R1

Clonality

Monoclonal

Clone

IL1-31-22.2.1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G purified

Concentration

1.45 mg/ml

Dilution

ELISA, IHC, IHC-P (10 μg/ml), RIA

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Not recommended for: WB. Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. ELISA: Can be used as capture reagents or second antibody at a concentration of 5 ug/ml. RIA: Concentrations of 20-30 ng/ml of these antibodies will bind radiolabeled This receptor in a radioimmunoassay format. Immunocytochemistry: This receptor can be detected on cultured cells using antibody concentrations of 10-100 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, RIA

Host or Source

Mouse

Preservative

PBS, pH 7.4

Isotype

IgG2b

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