CD40 Antibody
CD40 Antibody
Product Specifications
Product Name Alternative
CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5
Reactivity
Human
Target
CD40
Clonality
Monoclonal
Clone
LOB7/6
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A purified from tissue culture supernatant
Concentration
1 mg/ml
Dilution
Flo (20 μg/ml), IHC, IHC-Fr (1 - 10 μg/ml), IHC-P (10 μg/ml), IP
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.
Tested Applications
FC, IHC, IHC-Fr, IHC-P, IP
Host or Source
Mouse
Preservative
TBS, 0.09% Sodium Azide
Isotype
IgG2a
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