TRIM21/RO52 Antibody
TRIM21/RO52 Antibody
Product Specifications
Product Name Alternative
TRIM21, 52 kDa Ro protein, RING finger protein 81, RO52, Ro (SS-A), SSA1, Tripartite motif-containing 21, RNF81, SSA, Sicca syndrome antigen A, SS-A, Tripartite motif containing 21
Reactivity
Human, Monkey
Immunogen
Peptide with sequence CPLNIGSQGSTDY, from the C-Terminus of protein sequence according to NP_003132.2.
Target
TRIM21 / RO52
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IF (10 μg/ml), IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:16000), WB (0.2 - 1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IF, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
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