XRCC5/Ku80 Antibody
XRCC5/Ku80 Antibody
Product Specifications
Product Name Alternative
XRCC5, 86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, KARP-1, Ku autoantigen, 80kDa, NFIV, KARP1, Ku86, KUB2, TLAA, Nuclear factor IV, KU80, Thyroid-lupus autoantigen
Reactivity
Human
Immunogen
Peptide with sequence C-SLAKKDEKTDTLED, from the internal region of the protein sequence according to NP_066964.1.
Target
XRCC5 / Ku80
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (2.5 μg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
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