CR1/CD35 Antibody
CR1/CD35 Antibody
Product Specifications
Product Name Alternative
CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN
Reactivity
Human
Immunogen
Human acute monocytic leukaemia cells.
Target
CR1 / CD35
Clonality
Monoclonal
Clone
E11
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A purified
Concentration
1 mg/ml
Dilution
Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.
Tested Applications
FC, IHC, IHC-Fr, IHC-P
Host or Source
Mouse
Preservative
PBS
Isotype
IgG1
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