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TLR9 Antibody

TLR9 Antibody

Product Specifications

Product Name Alternative

TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9

Reactivity

Human, Mouse, Rat

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037) . Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) ; Chimpanzee, Marmoset (94%) ; Sheep, Goat, Zebu, Bovine, Horse (82%) .

Target

TLR9

Clonality

Monoclonal

Clone

26C593.2

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G purified

Concentration

1 mg/ml

Dilution

Flo, IHC, IHC-Fr (10 - 20 μg/ml), IHC-P (5 μg/ml), WB (1 - 3 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Tested Applications

FC, IHC, IHC-Fr, IHC-P, WB

Host or Source

Mouse

Preservative

PBS, 0.05% Sodium Azide

Isotype

IgG1, k

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