Endostatin Antibody
Endostatin Antibody
Product Specifications
Product Name Alternative
COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1 (XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1 (xviii), Knobloch syndrome, type 1
Reactivity
Human, Mouse, Rat
Immunogen
Full-length recombinant murine endostatin
Target
Endostatin
Clonality
Monoclonal
Clone
1837.46
Conjugation
Unconjugated
Purification
Thiophilic adsorption
Dilution
IHC, IHC-P (1:100), WB (1:100 - 1:500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Tested Applications
IHC, IHC-P, WB
Host or Source
Mouse
Preservative
50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.
Isotype
IgG
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