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Endostatin Antibody

Endostatin Antibody

Product Specifications

Product Name Alternative

COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1 (XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1 (xviii), Knobloch syndrome, type 1

Reactivity

Human, Mouse, Rat

Immunogen

Full-length recombinant murine endostatin

Target

Endostatin

Clonality

Monoclonal

Clone

1837.46

Conjugation

Unconjugated

Purification

Thiophilic adsorption

Dilution

IHC, IHC-P (1:100), WB (1:100 - 1:500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.

Tested Applications

IHC, IHC-P, WB

Host or Source

Mouse

Preservative

50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.

Isotype

IgG

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