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PAX7 Antibody

PAX7 Antibody

Product Specifications

Product Name Alternative

PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B

Reactivity

Human, Mouse

Immunogen

Synthetic peptide located between aa167-216 of human PAX7 (P23759, NP_002575) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%) ; Xenopus, Salmon, Zebrafish (92%) .

Target

PAX7

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Immunoaffinity purified

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), WB (0.05 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Tested Applications

IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS, 0.09% sodium azide, 2% sucrose

Isotype

IgG

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