PAX7 Antibody
PAX7 Antibody
Product Specifications
Product Name Alternative
PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B
Reactivity
Human, Mouse
Immunogen
Synthetic peptide located between aa167-216 of human PAX7 (P23759, NP_002575) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%) ; Xenopus, Salmon, Zebrafish (92%) .
Target
PAX7
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Immunoaffinity purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (5 μg/ml), WB (0.05 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
PBS, 0.09% sodium azide, 2% sucrose
Isotype
IgG
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