HEC1/NDC80 Antibody
HEC1/NDC80 Antibody
Product Specifications
Product Name Alternative
NDC80, HEC1, Kinetochore protein Hec1, Kinetochore associated 2, HEC, HsHec1, HsNDC80, KNTC2, TID3
Reactivity
Human
Immunogen
Peptide with sequence C-YEKKATLIKSSEE, from the C-Terminus of protein sequence according to NP_006092.1.
Target
HEC1 / NDC80
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
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