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ACTA2/Smooth Muscle Actin Antibody

ACTA2/Smooth Muscle Actin Antibody

Product Specifications

Product Name Alternative

ACTA2, ACTVS, Alpha-actin-2, Alpha-cardiac actin, AAT6, Actin, aortic smooth muscle, ACTSA, Smooth Muscle Actin, MYMY5, SMA

Reactivity

Bovine, Gallus, Human, Mouse, Rabbit, Rat

Immunogen

A synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. Cellular Localization: Cytoplasmic

Target

ACTA2 / Smooth Muscle Actin

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Immunoaffinity purified

Concentration

0.2 mg/ml

Dilution

IHC, IHC-P (5 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Positive control: Smooth muscle of arteries, gut wall, uterus and leiomyosarcoma.

Tested Applications

IHC, IHC-P

Host or Source

Rabbit

Preservative

10 mM PBS, pH 7.6, 15 mM Sodium Azide, 0.2% rAlbumin

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