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AHSG/Fetuin A Antibody

AHSG/Fetuin A Antibody

Product Specifications

Product Name Alternative

AHSG, A2HS, Alpha-2-Z-globulin, Fetuin, Fetuin-A, AHS, Alpha-2-HS-glycoprotein, Ba-alpha-2-glycoprotein, FETUA, HSGA

Reactivity

Human

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

Target

AHSG / Fetuin A

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Concentration

1 mg/ml

Dilution

ELISA (1:40000 - 1:200000), IHC, IHC-P (2.5 μg/ml), WB (1:10000 - 1:20000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Resuspension: Reconstitute in 500 μL of deionized water.. Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Isotype

IgG

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