HTR3A/5-HT3A Receptor Antibody
HTR3A/5-HT3A Receptor Antibody
Product Specifications
Product Name Alternative
HTR3A, 5-HT3-A, 5-HT3R-A, 5-HT3 receptor, 5HT3 serotonin receptor, 5-HT3A, 5-HT3a receptor, 5-hydroxytryptamine receptor 3, 5-HT-3, 5-HT3, 5-HT3R, 5HT3R, HTR3, Serotonin receptor 3A
Reactivity
Human, Rat
Immunogen
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949. Percent identity by BLAST analysis: Rat (100%) ; Mouse (88%) ; Hamster (81%) .
Target
HTR3A / 5-HT3A Receptor
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein G purified
Concentration
1 mg/ml
Dilution
IHC, IHC-P (5 μg/ml), WB (1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
PBS, 0.05% Sodium Azide
Isotype
IgG
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