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ACPP/PAP Antibody

ACPP/PAP Antibody

Product Specifications

Product Name Alternative

ACPP, ACP3, 5-NT, 5-nucleotidase, Acid phosphatase, prostate, ACP-3, Ecto-5-nucleotidase, PAP, Prostatic acid phosphatase, Thiamine monophosphatase, Prostatic acid phosphotase, TMPase

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length protein of human TMPase

Target

ACPP / PAP

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Immunoaffinity chromatography

Concentration

1 mg/ml

Dilution

IHC-P (1:100), WB (1:500 - 1:2000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Western blot analysis of TMPase expression in MCF7 (A) whole cell lysates. Immunohistochemical analysis of TMPase staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Tested Applications

IHC-P, WB

Host or Source

Rabbit

Preservative

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

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