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LRP2/Megalin Antibody

LRP2/Megalin Antibody

Product Specifications

Product Name Alternative

LRP2, DBS, Glycoprotein 330, gp330, Megalin, LRP-2, Calcium sensor protein

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length protein of human LRP2

Target

LRP2 / Megalin

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Immunoaffinity chromatography

Concentration

1 mg/ml

Dilution

IHC-P (10 μg/ml), WB (1:500 - 1:2000)

References & Citations

Manuel Johanns et al. Cellular uptake of proMMP-2:TIMP-2 complexes by the endocytic receptor megalin/LRP-2 Sci Rep, 7 (1), 4328 (2017)

Pubmed id

28659595

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Western blot analysis of LRP2 expression in mouse lung (A) whole cell lysates. Immunohistochemical analysis of LRP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Tested Applications

IHC-P, WB

Host or Source

Rabbit

Preservative

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

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