TFE3 Antibody
TFE3 Antibody
Product Specifications
Product Name Alternative
TFE3, BHLHe33, Transcription factor E3, RCCX1, TFEA, RCCP2
Reactivity
Human
Immunogen
Recombinant human TFE3.
Target
TFE3
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A/G purified
Dilution
ELISA (1:100 - 1:200), IHC-P (1:100 - 1:200), WB (1:100 - 1:200)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Tested Applications
ELISA, IHC-P, WB
Host or Source
Mouse
Preservative
Tris, pH 7.3-7.7, 1% rAlbumin, < 0.1% Sodium Azide
Isotype
IgG1
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