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TFE3 Antibody

TFE3 Antibody

Product Specifications

Product Name Alternative

TFE3, BHLHe33, Transcription factor E3, RCCX1, TFEA, RCCP2

Reactivity

Human

Immunogen

Recombinant human TFE3.

Target

TFE3

Clonality

Monoclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A/G purified

Dilution

ELISA (1:100 - 1:200), IHC-P (1:100 - 1:200), WB (1:100 - 1:200)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.

Tested Applications

ELISA, IHC-P, WB

Host or Source

Mouse

Preservative

Tris, pH 7.3-7.7, 1% rAlbumin, < 0.1% Sodium Azide

Isotype

IgG1

Frequently Asked Questions

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