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NOS1/nNOS Antibody

NOS1/nNOS Antibody

Product Specifications

Product Name Alternative

NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

Reactivity

Canine, Equine, Human, Mammal, Monkey, Mouse, Rabbit

Immunogen

Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS) . Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Dog, Bat, Elephant, Horse, Rabbit (100%) ; Rat, Chicken (92%) ; Xenopus, Zebrafish (83%) .

Target

NOS1 / nNOS

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Immunoaffinity purified

Concentration

1 mg/ml

Dilution

ICC, IHC, IHC-P (5 μg/ml), IP, WB

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Tested Applications

ICC, IHC, IHC-P, IP, WB

Host or Source

Rabbit

Preservative

PBS, pH 7.2, 0.02% Sodium Azide, 50% Glycerol

Isotype

IgG

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