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RAMP3 Antibody

RAMP3 Antibody

Product Specifications

Product Name Alternative

RAMP3

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length protein of human RAMP3

Target

RAMP3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Immunoaffinity chromatography

Concentration

1 mg/ml

Dilution

ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Western blot analysis of RAMP3 expression in MCF7 (A), mouse brain (B), mouse heart (C), rat brain (D), rat lung (E) whole cell lysates. Immunofluorescent analysis of RAMP3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Tested Applications

ICC, IF, IHC-P, WB

Host or Source

Rabbit

Preservative

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

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