RAMP3 Antibody
RAMP3 Antibody
Product Specifications
Product Name Alternative
RAMP3
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length protein of human RAMP3
Target
RAMP3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Immunoaffinity chromatography
Concentration
1 mg/ml
Dilution
ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Western blot analysis of RAMP3 expression in MCF7 (A), mouse brain (B), mouse heart (C), rat brain (D), rat lung (E) whole cell lysates. Immunofluorescent analysis of RAMP3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Tested Applications
ICC, IF, IHC-P, WB
Host or Source
Rabbit
Preservative
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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