IL6/Interleukin 6 Antibody

Product Specifications

Product Name Alternative

IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

Reactivity

Human

Immunogen

Purified recombinant fragment of human IL-6 expressed in E. coli.

Target

IL6 / Interleukin 6

Clonality

Monoclonal

Clone

6D9A1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Ascites

Dilution

ELISA (1:10000), IHC, IHC-P (1:200), WB (1:100 - 1:500)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:200.