PAX3 Antibody
PAX3 Antibody
Product Specifications
Product Name Alternative
PAX3, CDHS, Paired domain gene 3, Paired box protein Pax-3, Paired domain gene HuP2, Waardenburg syndrome 1, WS1, HUP2, Paired box 3, Paired box homeotic gene 3, WS3
Reactivity
Human, Mouse
Immunogen
Synthetic peptide located between aa400-449 of human PAX3 (P23760, NP_852122) . Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%) .
Target
PAX3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Immunoaffinity purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (5 μg/ml), WB (0.2 - 1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
PBS, 0.09% sodium azide, 2% sucrose
Isotype
IgG
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