NCS1/Neuronal Calcium Sensor Antibody
NCS1/Neuronal Calcium Sensor Antibody
Product Specifications
Product Name Alternative
NCS1, Frequenin (Drosophila) homolog, FREQ, FLUP, Frequenin homolog, Neuronal calcium sensor 1, Frequenin homolog (Drosophila), Frequenin-like protein, NCS-1
Reactivity
Human, Mouse, Rat
Immunogen
Frequenin (recombinant from Mouse with extensive post-translational modifications)
Target
NCS1 / Neuronal Calcium Sensor
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Affinity chromatography
Concentration
1 mg/ml
Dilution
ELISA (1:10000 - 1:50000), IF (1:100 - 1:2500), IHC, IHC-P (5 μg/ml), WB (1:500 - 1:2000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
ELISA, IF, IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Isotype
IgG
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