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BBC3/PUMA Antibody

BBC3/PUMA Antibody

Product Specifications

Product Name Alternative

BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA, PUMA alpha

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to 14 amino acids near the C-Terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the last 50 amino acids of PUMA.

Target

BBC3 / PUMA

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Peptide Column Affinity Chromatography

Concentration

1 mg/ml

Dilution

ELISA, ICC, IF, IHC, IHC-P (10 μg/ml), WB (1 - 2 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: PUMA antibody can be used for detection of PUMA by Western blot at 2 ug/mL. Antibody can also be used for immunocytochemstry at 1 ug/mL. For immunofluorescence start at 2 ug/mL. Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 23 kDa

Tested Applications

ELISA, ICC, IF, IHC, IHC-P, WB

Host or Source

Rabbit

Preservative

PBS containing 0.02% Sodium Azide

Isotype

IgG

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