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NR4A1/NUR77 Antibody

NR4A1/NUR77 Antibody

Product Specifications

Product Name Alternative

NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide from human NR4A1 / NUR77.

Target

NR4A1 / NUR77

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G purified

Concentration

1 mg/ml

Dilution

ChrIP (1:10 - 1:500), ICC, IF, IHC, IHC-P (20 - 40 μg/ml), IP (1:10 - 1:500), WB (1:500 - 1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20-40 ug/ml.

Tested Applications

ChIP, ICC, IF, IHC, IHC-P, IP, WB

Host or Source

Rabbit

Preservative

PBS, 0.05% Sodium Azide

Isotype

IgG

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